The working gas in the glove box is between the chamber and the purification column (anhydrox adsorber), and under the control and monitoring of the PLC, a closed cycle is performed by a pipe, a circulating fan or the like. The working gas is circulated through the purified column, and the moisture and oxygen are adsorbed, and then return to the chamber.

With the transition of the cycle time, the oxygen content in the working gas in the chamber is gradually reduced, and finally less than 1 ppm indicators. The purification column adsorbs saturated after a certain period of time, and can be regenerated and reused.